Onder 2.9 Gerechtshof Den Haag 16 februari 2016

Sprague-Dawley rats, which were in free access to ordinary dietes containing 2% cholestyramine and water for 2 weeks, were used for the preparation of rat liver microsomes. The thus obtained microsomes were then purified according to the manner described by Kuroda et al., Biochem. Biophys. Act, 486 , 70 (1977). The microsomal fraction obtained by centrifugation at 105000 x g was washed once with a buffered solution containing 15 mM nicotinamide and 2 mM magnesium chloride (in a 100 mM potassium phosphate buffer, pH 7.4). It was homogenized with a buffer containing nicotinamide and magnesium chloride at the same weight as the liver employed. The thus obtained homogenate was cooled down and kept at -80 ° C.

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