Citaat | Parket bij de Hoge Raad 24 november 2017

Onder 1.8 Parket bij de Hoge Raad 24 november 2017

Een alinea uit Parket bij de Hoge Raad 24 november 2017, ECLI:NL:PHR:2017:1282.

Sprague-Dawley rats, which were in free access to ordinary dietes containing 2% cholestyramine and water for 2 weeks, were used for the preparation of rat liver microsomes. The thus obtained microsomes were then purified according to the manner described by Kuroda et al., Biochem. Biophys. Act, 486, 70 (1977). The microsomal fraction obtained by centrifugation at 105000 x g was washed once with a buffered solution containing 15 mM nicotinamide and 2 mM magnesium chloride (in a 100 mM potassium phosphate buffer, pH 7.4). It was homogenized with a buffer containing nicotinamide and magnesium chloride at the same weight as the liver employed. The thus obtained homogenate was cooled down and kept at -80 ° C.

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