Citaat | Rechtbank Den Haag 16 januari 2019

Onder 2.9.2. Rechtbank Den Haag 16 januari 2019

Een alinea uit Rechtbank Den Haag 16 januari 2019, ECLI:NL:RBDHA:2019:321.

Mice. Female CD I nu/nu mice were purchased from Charles River Laboratories (Wilmington, MA, USA). Female DBA/2 mice were purchased from Taconic (Germantown, NY, USA). Mice weighed 20 to 25 grams at the beginning of the studies. Mice were housed in temperature and humidity controlled rooms. Mice were fed either standard laboratory rodent chow (Purina Chow #5001) or folic acid-deficient diet containing 1% succinylsulfathiazole (Purina Chow #5831C-2); both diets were purchased from Ralston Purina Co. (St. Louis, MO, USA). The average content of folates from natural sources in both diets was found to be 0.03 ppm, whereas the standard diet was analyzed to contain 7.3 ppm of added folic acid. It was estimated that mice on a standard diet ingested 1 to 2 mg/kg/day of folates, while mice on a low folate diet ingested 0.001 to 0.008 mg/kg/day. In some studies, mice received solubilized folic acid once a day by oral gavage. Food and water were provided ad libitum.

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